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. Author manuscript; available in PMC: 2011 Nov 19.
Published in final edited form as: Nature. 2011 Apr 20;473(7347):372–375. doi: 10.1038/nature09938

Figure 3. Neuropsin regulates the dynamics of EphB2/NR1 interaction and controls the expression of Fkbp5.

Figure 3

(a, b) EphB2 immunoprecipitation (before and after 15 minutes of restraint stress) from amygdalae revealed dissociation of EphB2/NR1 complexes in wild-type (F(3, 19)=4.2; p<0.05), but not neuropsin−/− mice. EphB2/NR1 dissociation was restored in neuropsin−/− mice by intraamygdalar neuropsin injections (F(3, 13)=4.7; p<0.05). Microarray analysis of wild-type and neuropsin−/− amygdalae revealed differential expression of Fkbp5 (heatmap in c, Suppl. Fig. 10). Exon-specific Fkbp5 probes showed an upregulation of the whole transcript (d) confirmed by qRT-PCR (e; F(3, 12)=72.15; p<0.001). qRT-PCR revealed attenuated stress-induced upregulation of Fkbp5 in neuropsin−/− mice (e; p<0.01 for wild-type after stress vs. neuropsin−/− after stress) rescued by intraamygdalar neuropsin injections (F(3, 14)=9.2; p<0.01). (f, g) Fkbp51 protein levels were upregulated in wild-type mice (F(3, 14)=8.95; p<0.001) but not in neuropsin−/− mice by stress. (h) Neuropsin-mediated upregulation of Fkbp5 in amygdala neuronal cultures (F(4, 29)=19.04; p<0.0001) was blocked by anti-EphB2 antibody and mimicked by stimulation of NMDA receptors (i; p<0.05). *p<0.05; **p<0.01; ***p<0.001. Results are shown as mean±SEM. NP- neuropsin. Digits inside columns indicate n number.