Activity of wild-type, alpha-null, gamma-null and double-null mice in the non-anxiogenic open field and home cage. (A) Double-null mice were significantly hyperactive in the non-anxiogenic open field compared to wild-type mice (one-way ANOVA, wild-type n = 10, alpha-null n = 10, gamma-null n = 10 and double-null n = 15 F3,41, = 3.56, P = 0.022; Bonferroni post-tests *P = 0.039 compared to wild-type). (B) There was no significant effect of genotype on the rate of rearing in the non-anxiogenic open field (one-way ANOVA, wild-type n = 10, alpha-null n = 10, gamma-null n = 10 and double-null n = 15 F3,41 = 1.42 P = 0.251). (C) There were no significant differences between any of the lines of mice in activity in the home-cage (one-way ANOVA, wild-type n = 10, alpha-null n = 10, gamma-null n = 10 and double-null n = 15 F3,41 = 0.026, P = 0.994).