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. Author manuscript; available in PMC: 2011 Jul 28.
Published in final edited form as: J Biol Chem. 2007 Aug 28;282(44):32298–32310. doi: 10.1074/jbc.M703451200

FIGURE 4. No metal enhances expression from a KmtR-regulated promoter in a mycobacterium grown in Middlebrook 7H9 medium.

FIGURE 4

A and B, β-galactosidase activity was measured in M. smegmatis mc2155 containing kmtR and Pcdf fused to lacZ (in pJEM15kmtR-Pcdf) grown with no metal supplement and maximum permissive concentrations of Zn(II) (70 μm), Co(II) (15 μm), Ni(II) (35 μm), Cd(II) (75 nm), Cu(II) (50 μm), Pb(II) (10 μm), Ag(I) (0.75 μm), Bi(III) (0.75 μm), or Mn(II) (0.75 μm) (A), or up to inhibitory [Zn(II)] (B). Inset, growth (A595) of cultures against added [Zn(II)]. C, microtiter plate assays of Zn(II)-PAR formation (400 μm PAR), measured at 492 nm and zeroed against apo-PAR, as a function of [Zn(II)] in the absence (closed symbols) or presence (open symbols) of KmtR (2 μm).