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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Immunol. 2011 May 23;187(1):200–211. doi: 10.4049/jimmunol.1100647

Figure 5. Concentration-dependent stimulation of patient peripheral blood mononuclear cells with piperacillin and characterization of the major antigen formed in cell culture.

Figure 5

(A) Concentration-dependent piperacillin-specific proliferation of PBMC from hypersensitive patients. Proliferative responses were analyzed by incorporation of [3H]thymidine of the final 16 h of the experiment. (B) Analysis of total levels of albumin binding with piperacillin concentrations associated with a significant lymphocyte proliferative response after 1-120 h. (C) Epitope profile showing the lysine residues of albumin modified with the cyclized and hydrolyzed piperacillin haptens in culture medium.