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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Immunol. 2011 May 23;187(1):200–211. doi: 10.4049/jimmunol.1100647

Figure 7. Stimulation of patient peripheral blood mononuclear cells and T-cell clones with a synthetic piperacillin albumin conjugate.

Figure 7

(A) Epitope profile of the piperacillin-albumin conjugate, derived from MRM-MS analysis of modified tryptic peptides. Specific proliferation of (B) PBMC and (C) T-cell clones from hypersensitive patients with the synthetic albumin conjugate. Unconjugated albumin subjected to the same extraction protocol as the piperacillin albumin conjugate was used as a control in each experiment. Proliferation in cultures containing unconjugated albumin were consistently less than 2000 cpm and no significant difference was observed when unconjugated albumin and medium controls were compared.