FIG. 2.

Effect of increasing concentrations of boldine on mRNA levels of (A) adiponectin and its transcription regulators, (B) PPARγ and (C) C/EBPα, in the presence of H₂O₂. Differentiated 3T3-L1 adipocytes were treated with H₂O₂ (100 μM) in the absence or presence of 5–100 μM boldine for 24 hours. After treatments, RT-qPCR was performed on isolated mRNA. The mRNA results are expressed as differences in fold change in antioxidant treated cells compared to vehicle controls. (D) The levels of secreted adiponectin protein were measured using Western blot analysis of the lyophilized cell supernatant from antioxidant-treated cells and are expressed as the percentage of their immunoblot intensity relative to vehicle controls. Data are mean ± SEM values for three independent experiments performed in triplicate. *P ≤ .05, **P ≤ .01 versus control.