FIG. 5.

Effect of boldine on PPRE-promoter activity. 3T3-L1 preadipocytes transfected with a PPRE-luciferase reporter construct was exposed to boldine (10 μM), the PPARγ agonist 15-PGJ2 (1 μM), or boldine (10 μM) + 15-PGJ2 (1 μM). PPRE-luciferase activity was measured as a change in chemiluminesence in a luminometer after a 24-hour treatment. The results are expressed as change in relative luminescence units (RLU) in treated cells compared to untreated controls. Data are mean ± SEM vales for five independent experiments performed in triplicate. *P ≤ .05 versus control.