Table 2.
Target amplified | ||
---|---|---|
Degenerate primers for cmeB of C. upsaliensis, C. lari, and C. fetus | ||
cmeB1213F | GGDATHGTYGTAGATGATGC | cmeB |
cmeB2817R | GCRAAYTCWACRATYAARATHGCATT | cmeB |
Mutagenesis primers | ||
chlU | ACGGATCCAAAGAGTGACCGCCGAGA (BamHI)a | cat cassette |
chlL | ACTCTAGACAGTGCGACAAACTGGGA (XbaI) | cat cassette |
kanU | ATGGATCCTGCAAGGAACAGTGAAT (BamHI) | kanr cassette |
kanL | ATTCTAGAGCGATGAAGTGCGTAAG (XbaI) | kanr cassette |
99UBF | CAATCCGTTAGTACAATGAG | C. coli cmeB |
99UBR | ACGGATCCCATATTATCTGCGCCATTGA (BamHI) | C. coli cmeB |
99LBF | GGTCTAGAGGAGTGGATCAGCTTATCAA (XbaI) | C. coli cmeB |
99LBR | GTATTCTCCTACTGCTCCTA | C. coli cmeB |
44BUF | CAGCTTGTAAGCGATTATGA | C. upsaliensis cmeB |
44BUR | ACGGATCCAGCGATAACTTCTAGTATGC (BamHI) | C. upsaliensis cmeB |
44BLF | ACTCTAGAAGCTAGTATGGCTCTAATCG (XbaI) | C. upsaliensis cmeB |
44BLR | CTAAGGTAGAAGCAGCTATC | C. upsaliensis cmeB |
LarUF | TCTAGCTTGTGCTTCGCTTA | C. lari cmeB |
LarUR | TTGGATCCGAAGAGCTTGAGGTTGAATC (BamHI) | C. lari cmeB |
LarLF | AATCTAGAGGCCATTGGTATTGTGGTTG (XbaI) | C. lari cmeB |
LarLR | GTGCAGGATCACCTTGAACT | C. lari cmeB |
FetUF | TATGCCAGTAACTGTTATGC | C. fetus cmeB |
FetUR | ACGGATCCTAGTATCGGCTATAGTCTGT (BamHI) | C. fetus cmeB |
FetLF | ACTCTAGATGTTCGCACTTATACTAGCC (XbaI) | C. fetus cmeB |
FetLR | TGATACTACCGAGTCTAACG | C. fetus cmeB |
Restriction sites are underlined and labeled in parentheses.