Table 2.
Key Polymerase Chain Reaction Primers Used in This Study
| Target amplified | ||
|---|---|---|
| Degenerate primers for cmeB of C. upsaliensis, C. lari, and C. fetus | ||
| cmeB1213F | GGDATHGTYGTAGATGATGC | cmeB |
| cmeB2817R | GCRAAYTCWACRATYAARATHGCATT | cmeB |
| Mutagenesis primers | ||
| chlU | ACGGATCCAAAGAGTGACCGCCGAGA (BamHI)a | cat cassette |
| chlL | ACTCTAGACAGTGCGACAAACTGGGA (XbaI) | cat cassette |
| kanU | ATGGATCCTGCAAGGAACAGTGAAT (BamHI) | kanr cassette |
| kanL | ATTCTAGAGCGATGAAGTGCGTAAG (XbaI) | kanr cassette |
| 99UBF | CAATCCGTTAGTACAATGAG | C. coli cmeB |
| 99UBR | ACGGATCCCATATTATCTGCGCCATTGA (BamHI) | C. coli cmeB |
| 99LBF | GGTCTAGAGGAGTGGATCAGCTTATCAA (XbaI) | C. coli cmeB |
| 99LBR | GTATTCTCCTACTGCTCCTA | C. coli cmeB |
| 44BUF | CAGCTTGTAAGCGATTATGA | C. upsaliensis cmeB |
| 44BUR | ACGGATCCAGCGATAACTTCTAGTATGC (BamHI) | C. upsaliensis cmeB |
| 44BLF | ACTCTAGAAGCTAGTATGGCTCTAATCG (XbaI) | C. upsaliensis cmeB |
| 44BLR | CTAAGGTAGAAGCAGCTATC | C. upsaliensis cmeB |
| LarUF | TCTAGCTTGTGCTTCGCTTA | C. lari cmeB |
| LarUR | TTGGATCCGAAGAGCTTGAGGTTGAATC (BamHI) | C. lari cmeB |
| LarLF | AATCTAGAGGCCATTGGTATTGTGGTTG (XbaI) | C. lari cmeB |
| LarLR | GTGCAGGATCACCTTGAACT | C. lari cmeB |
| FetUF | TATGCCAGTAACTGTTATGC | C. fetus cmeB |
| FetUR | ACGGATCCTAGTATCGGCTATAGTCTGT (BamHI) | C. fetus cmeB |
| FetLF | ACTCTAGATGTTCGCACTTATACTAGCC (XbaI) | C. fetus cmeB |
| FetLR | TGATACTACCGAGTCTAACG | C. fetus cmeB |
a
Restriction sites are underlined and labeled in parentheses.