FIG. 2.

Effect of inulin on glucose uptake is dependent upon both AMPK and Akt in C2C12 myotubes. C2C12 cells were pretreated with or without 20 μM compound C (Com.C) or 25 μM LY24002 for 30 minutes and then treated with 1 mg/mL inulin for 1 hour. (A) Protein extracts were prepared and subjected to western blot analysis using anti-phosphospecific AMPK-Thr¹⁷² (p-AMPK), total AMPK (AMPK-α), anti-phosphospecific Akt-Ser⁴⁷³ (p-Akt), and total Akt (AKT) antibodies. Experiments were repeated three times with similar results, and a representative result is shown. (B) Glucose uptake was measured as described in Materials and Methods. Data are mean ± SD values for two independent assays performed in triplicate. *P < .05 versus inulin alone.