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. Author manuscript; available in PMC: 2011 Jul 28.
Published in final edited form as: Curr Biol. 2008 Nov 25;18(22):1785–1791. doi: 10.1016/j.cub.2008.11.007

Figure 4. The N-terminal tail of Hec1 is necessary for establishing cold-stable microtubule attachments.

Figure 4

(A) Cells were incubated in ice-cold media for 10 min before fixation and immunofluorescence. Representative cells from each mitotic phase are shown as indicated. Arrows point to kinetochores that are enlarged below each merge. Bar = 5μm and 1μm (B) 10 kinetochores from 5 or more cells (n>50) were scored for associated microtubules in two independent assays and the mean percentage in early prometaphase (EP), late prometaphase (LP), and metaphase (M) was plotted. Error bars indicate standard deviation.