Figure 5.

Concentration at cell surface as a function of x for cases 1, 2, and 3, in units of nmol∕cm³ with (a) Q_I=2.5 nL∕s and Q_II=25 nL∕s, and (b) Q_I=2.5 nL∕s and Q_II=125 nL∕s. By increasing the flow rate in the upper chamber by a factor of 5, the concentration profile becomes more uniform. In particular, for the high uptake rate and confluent seeding case (Case 3), increasing the flow rate in the upper chamber prevents depletion along the length of channel caused by high uptake of oxygen. For both (a) and (b), the shear imparted on the cell culture is the same (τ=0.3 dyne∕cm²).