Figure 4.

Epitope mapping of 17A12 using synthetic peptides. An overlapping peptide array was constructed and used to precisely map the 17A12 binding site. Peptides were synthesized onto cellulose membranes as either the full 18-amino acid peptide identified from Figure 3 or decapeptides with one amino acid overlap. The cellulose membrane was exposed to 17A12 or 10D11 and then processed as a standard immunoblot. The reactive peptides, which comprise the epitope, are highlighted. The antibody used is indicated in the right margin.