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. 2011 Jul 26;2:163. doi: 10.3389/fmicb.2011.00163

Figure 5.

Figure 5

MAP1025 reacts with MAb 17A12. SDS-PAGE and immunoblot analysis of the MAP1025 recombinant fusion protein. The MBP-MAP1025 fusion protein migrates at approximately 70 kDa, which agrees with predicted sizes (25 kDa for MAP1025 plus 42 kDa for MBP). The MBP-LacZ control was loaded identically for both the SDS-PAGE and immunoblot; however, MBP-MAP1025 was diluted 1:2,000 for the immunoblot and 1:2 for SDS-PAGE. Note that the antibody only reacts with MAP1025 and not the MBP affinity tag. Kilodalton size standards are located in the left margin. Lanes: 1, protein size standards; 2, MBP-MAP1025; 3, MBP-LacZ.