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. 2011 Jul 26;2:163. doi: 10.3389/fmicb.2011.00163

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Copyright © 2011 Bannantine, Stabel, Lamont, Briggs and Sreevatsan.

This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.

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Sequence alignment of the 17A12 epitope region reveals a non-synonymous SNP in genomic DNA. (A) Alignment of 19 amplified products from mycobacterial genomic DNA were compared with the 17A12 epitope. The polymorphic nucleotide is shown in red, which results in an amino acid change from proline to histidine. (B) Sequence chromatogram of selected mycobacterial templates reveals sequence quality at the site of the C → A polymorphism. The seven codons that encode the 17A12 epitope are shaded in gray. Arrows point to the adenine nucleotide SNP present in the first codon of the epitope.