Table A2.
Primers used in this study.
| Construct | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|
| UP1-#1 | ATCCTCTAGAGGTGATCTCAATCCTGCTGCG | GCGCAAGCTTCTACACCGCCCGGGTGCAGGC | 1182 |
| UP1-#3 | ATCCTCTAGAGGTGATCTCAATCCTGCTGCG | GCGCAAGCTTACCGATCATCGGTGATCCGT | 351 |
| UP1-#4 | ATCCTCTAGAGGTGATCTCAATCCTGCTGCG | GCGCAAGCTTAGTGGTTACCGCCGACGGGGCG | 927 |
| UP1-#6 | ATCCTCTAGAGATCAGCGACTCCCGGGTGCC | GCGCAAGCTTCTACACCGCCCGGGTGCAGGC | 1206 |
| UP1-#7 | ATCCTCTAGAGATCAGCGACTCCCGGGTGCC | GCGCAAGCTTACCGGCGCCGCTGCCGGTTCGC | 1077 |
| UP1-#8 | ATCCTCTAGAGATCAGCGACTCCCGGGTGCC | GCGCAAGCTTACCGATCATCGGTGATCCGT | 375 |
| UP1-#10 | ATCCTCTAGAGTCGACCACCGCCCCGTCGGC | GCGCAAGCTTACCGGCGCCGCTGCCGGTTCGC | 156 |
| UP1-#11 | ATCCTCTAGACACTTCGGCGGCAAGGACTTT | GCGCAAGCTTACACCGCCCGGGTGCAGGC | 258 |
| UP1-#12 | ATCCTCTAGACACACTGGCCCCGGCCGGCAA | GCGCAAGCTTACCCGCCCGGGTGCGGCTGCGC | 156 |
| UP1-#16 | ATCCTCTAGACTCCAGGAAGCTGTGCACGTG | GCGCAAGCTTAGTGGTTACCGCCGACGGGGCG | 1146 |
| UP1-#21 | ATCCTCTAGACGCCAGGATCGTCGAGAGCAC | GCGCAAGCTTACCGATCATCGGTGATCCGT | 465 |
| UP1-#23 | ATCCTCTAGACAACAGCTCCACCTCCGTCAG | GCGCAAGCTTACCGGCGCCGCTGCCGGTTCGC | 621 |
| UP1-#24 | ATCCTCTAGACAACAGCTCCACCTCCGTCAG | GCGCAAGCTTCTACACCGCCCGGGTGCAGGC | 750 |
| MAP1025 | ATCCTCTAGATTGCCCATGACCGATCAACCGC | GCGCAAGCTTCTAGCTCGGCGGGCTTTCGGAG | 726 |
| MAP3422c | ATCCTCTAGACCGGCGCCGCTGCCGGTTCGC | GCGCAAGCTTCATGGACCTGGGTCGTCGAG | 873 |
| UpET1 | CACCATGGATCAGCGACTCCCGGGT | CTACACCGCCCGGGTGCAGGCCGA | 1209 |
| UpET2 | CACCCTGGGCAAGCAGCTGCAGCGG | CTACACCGCCCGGGTGCAGGCCGA | 915 |
| UpET4 | CACCCTGTCATGGACCTGGGTCGTC | CTACACCGCCCGGGTGCAGGCCGA | 999 |
| UpET5 | CACCTTGCCGTCGCACACGCCACCA | CTACACCGCCCGGGTGCAGGCCGA | 1047 |
| UpET6 | CACCGTGCTGGTTCCAATCCAGCAG | CTACACCGCCCGGGTGCAGGCCGA | 1158 |
All primers are listed 5′–3′. Nucleotides specific for cloning purposes are underlined.