Skip to main content
. 2011 Aug 3;101(3):651–661. doi: 10.1016/j.bpj.2011.06.025

Figure 1.

Figure 1

U-type inactivation of wt Kv2.1. (A, Top) Schematic of the three-pulse (3P) protocol used to study Kv2.1 inactivation. The voltage for the 15 s P2 was increased in 20-mV increments, whereas P1 and P3 were at +60 mV for 300 ms. (Bottom) Macroscopic currents recorded using the 3P protocol from an oocyte expressing wt Kv2.1 channels. Shown are nine superimposed traces obtained in ND96 solution. The sweep interval was 60 s. (B) The inactivation-voltage relationship for wt Kv2.1 was determined by taking the ratio of current (I3/I1) measured during P3 and P1 and plotting it against the P2 voltage. (Data points) Mean ± se; where not visible, error bars are hidden within symbols. n = 7. (Solid line) Fit of the descending portion of the inactivation-voltage relationship (i.e., between −100 and +20 mV) to a Boltzmann function. See Table 1 for a summary and the fit parameters.