Table 1.
Mutations in the S3-S4 and S5-P-loop linkers alter Kv2.1 inactivation
| Construct | V1/2 (mV) | k (mV) | Minimum I3/I1 | % Increase in I3/I1 at +60 mV∗ | n |
|---|---|---|---|---|---|
| wt | −21.0 ± 0.5 | 6.7 ± 0.3 | 0.24 ± 0.01 | 42.2 ± 6.3 | 7 |
| S3-S4 linker mutants | |||||
| N287C | −43.0 ± 4.3 | 16.6 ± 1.2† | 0.47 ± 0.02 | 36.6 ± 6.3 | 5 |
| V288C | −14.9 ± 2.7 | 11.4 ± 0.9† | 0.44 ± 0.02 | 26.6 ± 4.1 | 7 |
| R289C | −45.2 ± 2.6 | 17.8 ± 0.2† | 0.53 ± 0.02 | 27.0 ± 6.0 | 4 |
| R290C | −36.2 ± 2.3 | 14.1 ± 0.7† | 0.41 ± 0.03 | 69.8 ± 8.4 | 5 |
| V291C | −12.2 ± 1.8 | 6.1 ± 0.5 | 0.19 ± 0.03 | 52.6 ± 8.7 | 5 |
| V292C | −8.8 ± 3.7 | 15.0 ± 0.7† | 0.38 ± 0.04 | 32.2 ± 8.7 | 4 |
| S5-P-loop mutants | |||||
| K350C | −19.9 ± 2.3 | 11.2 ± 0.2† | 0.21 ± 0.02 | 33.6 ± 10.5 | 5 |
| D351C | −18.3 ± 0.6 | 9.4 ± 0.2 | 0.22 ± 0.02 | 28.5 ± 3.9 | 5 |
| E352C | −13.9 ± 0.9 | 9.9 ± 0.4 | 0.37 ± 0.03 | 6.6 ± 2.0† | 9 |
| D353C | −16.0 ± 2.1 | 8.1 ± 0.2 | 0.24 ± 0.03 | 43.2 ± 10.3 | 7 |
| D354C | −18.6 ± 0.9 | 6.7 ± 0.2 | 0.17 ± 0.01 | 66.6 ± 6.2 | 13 |
| T355C | −23.8 ± 1.6 | 7.9 ± 0.3 | 0.18 ± 0.03 | 58.8 ± 13.8 | 6 |
| K356C | −20.0 ± 1.3 | 6.4 ± 0.4 | 0.13 ± 0.01 | 75.3 ± 5.6 | 8 |
∗
Relative to the minimum I3/I1.
†
Significant difference (P < 0.05) from wt values, based on the results of a Kruskal-Wallis ANOVA on ranks test.