EM measurements on the effect of ionic strength, dextran concentration, and temperature on the ratio of small-square to basketweave (circles, A and B), A-lattice spacing (triangles, C and D), basketweave Z-lattice spacing (diamonds, C and D) and small-square Z-lattice spacing (squares, C and D). In A and C, solid symbols = standard ionic strength and open symbols = low ionic strength, which in C nearly or completely overlap. In A and C, dextran concentration and ionic strength were varied while temperature was held constant (38°). In B and D, temperature was varied while ionic strength (standard) and dextran concentration (3%) were held constant. (C) Left vertical axis represents actual EM measurements reported in Table 1, while right-hand axis represents the same values divided by a factor of 0.73 for comparison with previously published XRD data taken at 5°C (15). Corrected EM measurements for the A-lattice (triangles3 right-hand scale) closely match published XRD data (X, right-hand scale). Error bars represent standard deviation.