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Inactivation. (A) Voltage protocol: a P1 depolarization to +50 for 600 ms to activate the channels was followed by a repolarizing pulse to −90 mV for 60 ms to remove inactivation, then a P2 pulse to a range of potentials between −100 and +50 mV to allow direct observation of inactivation. (Inset) Details of P2 pulse. (B–F) The inactivation protocol was applied to all models.
