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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: J Immunol. 2010 Oct 25;185(11):7026–7036. doi: 10.4049/jimmunol.1001293

Figure 2. GM35 binding increases PMN adhesion to T84 and HT29 cells.

Figure 2

(A) T84 or (B) HT29 monolayers were treated with 10μg/ml GM35 or isotype control IgG1 before the addition of 5 × 105 BCECF labeled PMNs pretreated with 5μg/ml of indicated antibodies. Data shown are the % of total PMN remaining adherent following washing (n=3). (C) T84 cells were treated for 24h with 100U/ml IFNγ before cell lysis and western blotting with GM35. Data depicts representative results from n=3 blots. (D) T84 cells were incubated with 100U/ml IFNγ for 24 hours before treatment with 10μg/ml GM35 or isotype control antibody followed by the addition of 5 × 105 BCECF labeled PMNs. Data shown are % of total PMN remaining adherent following washing (n=3). Significance was defined at p<0.05 (*, p<0.05; **, p<0.01; ***, p<0.001).