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. Author manuscript; available in PMC: 2011 Jul 28.
Published in final edited form as: Development. 2007 Jul 11;134(16):2959–2968. doi: 10.1242/dev.006296

Figure 4. LHW gene and protein structure and behavior in two hybrid screen.

Figure 4

(A) LHW gene structure with exons represented as boxes and introns as lines. LHW coding region is in black. The location and nature of lhw mutant alleles is indicated above the exons. (B) LHW protein structure. Two domains conserved with other plant proteins are indicated as grey boxes. Part of the C-terminal conserved region resembles the bHLH domain of transcriptional regulators. A sequence alignment of the putative bHLH domain (boxed) is diagrammed for LHW and related proteins from Arabidopsis and rice. (C) Graphical representation of LHW protein fragments used in yeast two hybrid assay and their ability to dimerize with full length LHW. (D) GAL4 transcriptional activation activity of LHW variants; pACT is included as a negative control. GUS measurements based on 4 replicates/sample. Error bars ± SEM.