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. Author manuscript; available in PMC: 2012 Apr 8.
Published in final edited form as: Mol Cell. 2011 Apr 8;42(1):36–49. doi: 10.1016/j.molcel.2011.02.020

Figure 2. AKT activation fails to induce SAHF or the senescence-secretome.

Figure 2

(a) IMR90 fibroblasts were transduced with BRAFV600E, RASG12V, mAKT1 or a short hairpin that targets PTEN. Cells were drug selected, fixed and stained for SAHF, PML or HIRA foci. (b) and (c) One hundred cells from (a) were scored for HIRA foci or SAHF. Mean of 3 experiments with standard deviation. (d) RNA was harvested from mAKT1, RASG12V or control cells and assayed for expression of IL-6, IL-8, MMP-1 and MMP-3 by quantitative RT-PCR. (e) Expression profiling was performed on control, RASG12V or mAKT1 transduced IMR90. Heatmap of significantly upregulated (green) and downregulated (red) genes with GO classification “Inflammation” is shown.