Table 1.
Primers | Accession number | Sense (5′–3′) | Antisense (3′–5′) | T m (°C) |
---|---|---|---|---|
IKK | NM_010546 | CCAGACTCCAAGGTGGTGTT | TGCAGATCACAGGCAGAAAC | 60.0 |
TNF-α | NM_013693 | TGGCACAGCCAAG | GGGACCCCTGCTC | 52.36 |
TGF-β | NM_011577 | TTGCTTCAGCTCCACAGAGA | TGGTTGTAGAGGGCAAGGAC | 59.99 |
IL-1β | NM_008361 | GTGGCAGCTACCTGTGTCTT | GGAGCCTGTAGTGCAGTTGT | 57.96 |
IL-6 | NM_031168 | GGGAAATCGTGGA | AGGTTTGCCGAGT | 43.9 |
IL-10 | NM_010548 | CCAAGCCTTATCGGAAATGA | TTTTCACAGGGGAGAAATCG | 60.035 |
COX1 | BC023322 | AGAAACTGGTCTGCCTCA | AACCCACATCAAGGACTG | 54.02 |
COX2 | NM_011198 | AGCACCATTCTCCTTGAA | GTAGGCTGTGGATCTTGC | 54.0 |
PKC-θ | NM_008859 | AAGTGAGAAACCCCGGCTAT | AGGCAAATCCCTTCCAGTCT | 60.01 |
Perforin | NM_011073 | ACCCTGAATGGGCTCACA | GCAGCAGTCCTGGTTGGT | 57.0 |