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. Author manuscript; available in PMC: 2011 Jul 28.
Published in final edited form as: Proteins. 2010 Dec;78(16):3260–3269. doi: 10.1002/prot.22833

Table I.

Stability and Calcium Binding Parameters of Calbindin Variants

Variant Cm (M)
DG H2O(kcal mol−1)
m (kcal mol−1 M−1)
Ka,obs (M−1) n
Ka,obs (M−1) n
Apo Holo Apo Holo Apo Holo Pyrene BODIPY
Permuted calbindin 2.78 4.49 5.54 7.98 1.99 1.78 N.A.a N.A. N.A. N.A.
Permuted calbindin F66W 1.70 3.93 3.57 6.48 2.10 1.65 N.A. N.A. N.A. N.A.
Q65′ 2.65 5.64 3.90 7.95 1.47 1.41 5.50 × 105 2.43 8.55 × 105 2.75
(Q65′1W66) 2.51 4.78 3.67 3.02 1.55 0.63 3.88 × 105 1.76 5.99 × 105 2.65
(Q65′1W66′) 2.61 5.54 3.85 8.55 1.48 1.54 N.M.b N.M. 1.42 × 106 1.81

Standard deviations are: ±0.05 M (Cm), ± 0.4 kcal mol−1 (ΔGH2O), ± 0.1 kcal mol−1M−1 (m), ± 5 × 104 (Ka,obs), and ± 0.1 (n).

a

N.A., not applicable.

b

N.M., not measured due to insufficient amplitude.