Fig. 7.

Calcium oxalate crystals deposits identified using polarizing optics and Pizzolato staining of paraffin-embedded kidneys. (A) Kidney of a rat consuming only HLP. Polarized light showed a large number of CaOx crystal deposits. (B) Kidney of a HLP-consuming rat treated with apocynin. There is minimal CaOx crystal deposition. (C) Kidney of a rat consuming HLP. Results of Pizzolato staining are similar to that of examination using polarized light. (D) Kidney of a HLP-consuming rat treated with apocynin. Results of Pizzolato staining are similar to that of examination using polarized light. Crystal deposition is markedly reduced. Only a few crystals are present, mostly at corticomedullary junction.