Fig. 2.

HNE adduct formation is enhanced in the plasma of akr1b3-null mice. Western blots of plasma from NC and HF fed mice from WT and akr1b3-null mice were probed with anti-HNE antibodies. NC fed WT and akr1b3-null plasma served as respective controls. Western blots were developed using anti-HNE antibodies. Bar graphs show the intensity of the indicated anti-HNE normalized to Amido Black stains. Data are presented as mean ± S.E.M. *P < 0.01 vs WT NC and HF fed mice.