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AGEs and acrolein adduct formation is increased in the epididymal fat of HF fed mice. Cytosolic fractions of epididymal fat from WT and akr1b3-null HF and NC fed mice were probed with (A) anti-AGE antibody to identify AGEs and (B) anti-acrolein antibody. The membranes were visualized by chemiluminescence. Western blots were normalized by Amido Black stains *P < 0.05 compared with WT (NC).
