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. 2011 Jul 20;13(8):830–845. doi: 10.1093/neuonc/nor088

Fig. 1.

Fig. 1.

Glioblastoma multiforme (GBM) neuroglia (NG)-2+ cells show higher levels of cell proliferation compared with the GBM NG2− cells from the same tumor. Analysis of 8 GBM showed proliferative dominance by NG2+ cells (Supplementary Material, Table S1). Illustrative example from 1 cell line: (A) Normalized data from MTS assay shows higher signal in the GBM NG2+ cell cultures, indicating more robust cell growth (3 repeats per cell line). (B) Higher number of total cells in the GBM NG2+ cultures (3 repeats per cell line). (C) Ki67 staining of the fluorescent-activated cell–sorted GBM NG2+ and GBM NG2− cells (scale bars: 100 µm). (D) Quantification of the number of Ki67+ cells showing higher number in the GBM NG2+ cultures (3 repeats for each of 3 cell lines). (E) Quantification of the proportion of Ki67 cells showing higher proportion in the GBM NG2+ cultures. Note: Comparisons were made between NG2+ and NG2− populations within each tumor. Comparison between tumors was not made owing to heterogeneity between patients.