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Characterization of the PDZ ligand within the C-tail domain of the hIP. S.c Y187 (pACT2:PDZK1) or, as a control, S.c Y187 (pTD1-1) prey strains were mated with S.c AH109 bait strains transformed with pGBKT7.hIP299-386 subfragment with its wild-type (-C383SLC386, corresponding to the positions (P)0, P−1, P−2, and P−3 of its PDZ ligand, respectively) carboxyl-terminal residues, or the listed mutated variants, and as controls, p53 or transformed with the vector pGBKT7 alone. Data: n ≥ 3.
