Table 1.
Primers used in construction of DNA clones.
| DNA clones | Primer | Primer Sequences (5'-3')c | Primer location | Application |
|---|---|---|---|---|
| PCV2 | Ps1 | GCGAATTCAACCTTAACCTTTCTTATTCT | 1418-1446 nt | PCV2 genome |
| DNA clone | Ps2 | AAGAATTCTGGCCCTGCTCCC | 1407-1427 nt | |
| a Ps3 | GCTCTAGAATAACAGCACTGGAG | 1356-1378 nt | Duplicate | |
| a Ps4 | GAGGTACCCGTTTTCAGATATGAC | 1730-1753 nt | ||
| PCV1 | Pj3 | ACGGTACCCGAAGGCCGATTTGAA | 914-937 nt | PCV1 genome |
| DNA clone | Pj4 | TAGGTACCTCCGTGGATTGTTCTCC | 899-923 nt | |
| bEcoRV-f12 | GCGATATCATGGAGAAGAAGTTGTTGT | 649-675 nt | Duplicate | |
| bEcoRI-r12 | CCGAATTCTCTTTCACTTTTATAGGATG | 1721-1748 nt | ||
| PCV1-NLS2 | ClaI-f | CAATCGATAACGCCTCCTTGGATACGTCATC | 1694-1724 nt | PCV1 ΔNLS |
| DNA clone | BglII-r | GGAGATCTTCAATTCCCGCCTTTCTAG | 1566-1592 nt | |
| BamH-F | TAGGATCCCCGTGCCATTTTTCCTTC | 1594-1619 nt | PCV2 NLS | |
| MspI-r | CGTTACCGGAGAAGAAGACAC | 1696-1716 nt | ||
| PCV2-NLS1 | BalI-PCV2-F | CCTTGGCCACGTCATATCTGAA | 1720-1741 nt | PCV2 ΔNLS |
| DNA clone | BamH-PCV2-R | GAAAAATGGGATCCTCAACACC | 1594-1615 nt | |
| BglII-PCV1-F | TACAGATCTTTCGGCGCCA | 1588-1606 nt | PCV1 NLS | |
| BalI-PCV1-R | TATTGGCCAAGGAGGCGTTA | 1701-1720 nt |
a, Primers also used for amplification of duplicate in PCV2-NLS1 DNA clone construction.
b, Primers also used for amplification of duplicate in PCV1-NLS2 DNA clones construction.
c, The restriction enzyme sites were underlined.