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Primers and probes designed for Real-time PCR
| Primer | Sequence (5'-3') | Primer location | Targeting |
|---|---|---|---|
| Re-ORF22-F1 | CGCTCTGTGCCCTTTGAATAC | 1325-1347 nt | |
| Re-ORF22-R1 | GTGAGGGCTGTGGCCTTTGTTAC | 1450-1470 nt | PCV2 ORF2 |
| PCV2-probe1 | FAM-ATTCTGGCCCTGCTCCCCGAT-TAMRA | 1403-1423 nt | |
| Re-PCV1-F | AGGTGATGGGGTCTCTG | 1384-1400 nt | PCV1 ORF2 |
| Re-PCV1-R | TACCCCTACCTTTCCAATACTAC | 1433-1455 nt | |
| PCV1-FAM | FAM-ATTCATATTTAGCCTTTCTAAT-TAMRA | 1408-1429 nt |
