Figure 3. A link between the formation of SLDs and an elevated level of cellular PA.

A&B). Quantitation of cellular PA in WT and mutants. Cells were grown in SC medium to early stationary phase, harvested, and lyophilized. Lipids were extracted and PA levels were determined by LC-MS. *, p<0.05, compared to WT. C). Overexpression of PAH1 and DPP1 significantly reduces the formation of SLDs in fld1Δ, ino2Δ, and ino4Δ strains. Cells transformed with BG1805-PAH1, BG1805-DPP1 (both from Open Biosystems) or empty vector were cultured in synthetic galactose medium (2% galactose, 0.67% yeast nitrogen base, and amino acids) to stationary phase, stained with Nile red, and examined for the presence of SLDs.