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. 2011 Jul 28;7(7):e1002206. doi: 10.1371/journal.pgen.1002206

Figure 2. dMi-2 recruitment to HS genes requires PARP activity.

Figure 2

(A and B) ChIP analyses of dMi-2 binding to the hsp70 gene in Kc cells. (A) Upper panel: hsp70 gene and position of amplimers analysed (1: centred at -154, 2: +681). Middle panel: dMi-2 ChIP from cells treated with dsRNA against luciferase or dMi-2 as indicated. prom (amplimer 1): promoter; ORF (amplimer 2): open reading frame; NHS: non heat shock; HS: heat shock. Lower panel: Verification of RNAi knockdown by Western blot. (B) Upper panel: hsp70 gene and position of amplimers analysed (1: centred at -350; 2: -154; 3: +58; 4: +681; 5: +1702; 6: +2065; 7: +2549). Lower panel: dMi-2 ChIP from NHS (black graph) and HS (gray graph) cells. (C and D) Effect of elongation inhibitor DRB (C) and PARP inhibitor PJ34 (D) on dMi-2 recruitment to hsp70 gene. hsp70 gene and position of amplimers analysed are shown on top (1: centred at +58; 2: +681; 3: +1702; 4: +2549). Left panels: ChIP analyses of dMi-2 binding to hsp70 gene. Right panels: RT-QPCR analysis of hsp70 transcription. (D) Rightmost panel: anti-PAR Western blot of extracts from untreated and PJ34 treated Kc cells.