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. 2011 Jul 28;7(7):e1002206. doi: 10.1371/journal.pgen.1002206

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Murawska et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A and B) Upper panels: Schematic representations of the hsp70 and hsp83 genes. RT-QPCR amplimers, hsp70 cleavage site, hsp83 intron and transcriptional start sites (TSS) are shown. (A) Lower panel: RT-QPCR from control and transgenic larvae. The ratio between 3′ unprocessed and total hsp70 RNA was determined (hsp70: amplimer 2/amplimer 1). The ratio obtained for control larvae was set to 1, other ratios were expressed relative to this. (B) Lower panel: The ratio between unspliced and total hsp83 RNA was determined (hsp83: amplimer 1/amplimer 2) and plotted as in (A).