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. 2011 Jul 28;7(7):e1002205. doi: 10.1371/journal.pgen.1002205

Figure 1. The EAEC sci1 T6SS gene cluster is regulated by iron levels and the Fur repressor.

Figure 1

(A) β-galactosidase activity of a promoterless lacZ fusion (open symbols) and of the sci1-lacZ reporter fusion (closed symbols) upon addition of the iron chelator 2,2′-dipyridyl (dip; 100 µM, squares) in a EAEC wild-type (WT) strain (triangles: no dip added). (B) β-galactosidase activity of a promoterless lacZ fusion (white bars) and of the sci1-lacZ reporter fusion (black bars) after 120 minutes of culture (OD600nm = 0.8) upon a 30 min treatment with 2,2′-dipyridyl (+dip; 100 µM) or ethanol-carrier (-dip) in a WT strain or its isogenic fur mutant.