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. 2011 Jul 28;7(7):e1002205. doi: 10.1371/journal.pgen.1002205

Figure 6. GATC methylation influences Fur binding on fur1.

Figure 6

(A) Electrophoretic mobility shift assay of the non methylated or Dam-methylated (me-) sci1 or cir promoter using purified Fur (lanes 1 and 5, no protein; lanes 2 and 6, 2 nM; lanes 3 and 7, 5 nM; lanes 4 and 8-10, 20 nM). (B) Electrophoretic mobility shift assay of the non methylated or Dam-methylated (me-) fur1 sequence using purified Fur (lanes 1 and 6, no protein; lanes 2 and 7, 0.5 nM; lanes 3 and 8, 2 nM; lanes 4 and 9, 5 nM; lanes 5 and 10, 20 nM). Fur-DNA, (Fur)2-DNA complexes are indicated by *, and ** respectively.