Figure 2. Cell detachment induced by EDTA was inhibited in L6 cells by isoprenaline.

A) Differentiated L6 myotubes were kept in culture media (CM) or EDTA with or without 1 µM isoprenaline (ISO) for 20 min. The isoprenaline stimulated cells were pre-treated with this drug for 30 min before EDTA exposure. B) FITC-Phalloidin-stained L6 myotubes in CM, EDTA or EDTA+ISO. Representative pictures from 3–6 independent experiments.