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. 2011 Jul 28;6(7):e22448. doi: 10.1371/journal.pone.0022448

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Peris-Bondia et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The three panels show the distribution of events in FL8 PMT versus FL2 PMT. X and Y axis are defined in logarithmic arbitrary units. Gray histograms resume the distribution of events along FL2 PMT. Panel A shows unstained cells. Panel B shows cells stained with pyronin-Y with trigger in SS PMT; it is possible to appreciate the two populations of unstained and stained cells respectively (dotted black line separates the two populations). Panel C shows only pyronin-Y stained cells passing the threshold on FL2 PMT.