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. 2011 Jul 8;108(30):12473–12478. doi: 10.1073/pnas.1100432108

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Fig. 3. — Effects of RNA–Hfq interactions. A series of 19 RyhB mutants was constructed by random oligosynthesis. Each of these mutants has a number of bases in the linker region (positions 57–68 in Fig. S1) replaced by a different base. (A) The repressive effect of these mutant RyhBs on sodB-GFP expression (induced by 1 mM IPTG) is seen to correlate well with the energy cost () of keeping the 12-base linker region open. (B) The minimal free energy structure of the mutant RyhB-rh11 expressed in strain ZZS00-H11. This mutant gave strong repression (13.7×) despite a large value of . The structure of this mutant is predicted to have the interaction region completely open. The bases are colored by the same color scheme as that used in Fig. S1; the substituted bases are circled.

Inline graphic — Effects of RNA–Hfq interactions. A series of 19 RyhB mutants was constructed by random oligosynthesis. Each of these mutants has a number of bases in the linker region (positions 57–68 in Fig. S1) replaced by a different base. (A) The repressive effect of these mutant RyhBs on sodB-GFP expression (induced by 1 mM IPTG) is seen to correlate well with the energy cost () of keeping the 12-base linker region open. (B) The minimal free energy structure of the mutant RyhB-rh11 expressed in strain ZZS00-H11. This mutant gave strong repression (13.7×) despite a large value of . The structure of this mutant is predicted to have the interaction region completely open. The bases are colored by the same color scheme as that used in Fig. S1; the substituted bases are circled.