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Schematic of the reaction catalyzed by a tRNA-dependent aminoacyltransferase FemX and proposed function of PacB. FemX catalyzes the addition of Ala to a peptidylglycan precursor; PacB is predicted to add Ala or Gly (Ala shown) to mTyr2-DABA3-Ala4-CO-mTyr5.