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Characterization of PacB by LC-MS analysis. (A) Extracted ion chromatograms showing production of pacidamycin group of uridyl pentapeptides (1–3) and the hydrolytic intermediate 4. The calculated mass with a 10-ppm mass tolerance was used to create the extracted ion chromatograms. (B) Detection of PacH-bound biosynthetic intermediates with the incorporation of N-terminal Ala catalyzed by PacB. The intermediates were analyzed by FTMS after trypsin digestion of PacH.
