Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Characterization of PacB using the [14C]l-Ala substrate. (A) Autoradiograph of SDS-PAGE gel illustrating the covalent loading of [14C]l-Ala on dipeptidyl-S-PacH. ATP, m-Tyr, DABA, aatRS, and tRNA were present in all assays. Lanes 1–3 show reactions stopped at different time intervals (30, 60, and 120 min, respectively). Reactions in lane 4–7 were stopped after 120 min. (B) Time-course studies of the formation of [14C]Ala1-mTyr2-DABA3-S-PacH using various activated alanyl species.
