Skip to main content
. 2011 Jul 11;108(30):12325–12330. doi: 10.1073/pnas.1102789108

Fig. 1.

Fig. 1.

Reduced H4K16 acetylation in Zmpste24−/− cells. (A) Zmpste24+/+ and Zmpste24−/− MEFs at early passage (P3) (Upper panels) or late passage (P6) (Lower panels) were cultured for 2 d. SA β-gal activity was determined. (B and C) Histones were extracted from Zmpste24+/+ and Zmpste24−/− MEFs (P3) and analyzed by Western blotting using the antibodies indicated. (D) Graph shows normalized densitometric values of acetyl H4 and acetyl H4K16 against total H4 for three independently derived lines of MEFs in separate experiments. *P < 0.05 and **P < 0.01 (Student’s t test) when Zmpste24+/+ and Zmpste24−/− MEFs were compared. (E) Immunofluorescence staining of Zmpste24+/+ and Zmpste24−/− MEFs with lamin A/C (green) and anti-acetyl H4K16 (red) antibodies. (F) Histones were extracted from the liver of 1-mo-old mice and analyzed by Western blotting using the antibodies indicated.