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. 2011 Jul 28;6(7):e22733. doi: 10.1371/journal.pone.0022733

Figure 6. Morphology and LGR5 reactivity of xenografts tumours.

Figure 6

A) Haematoxilin and eosin staining of frozen sections from representative tumours generated from parental LIM1899, LIM1899 transfected with siRNA to LGR5 or LIM1899 transfected with pTune-LGR5 construct. Brightfield images were acquired on a Nikon 90i microscope with a 20× lens. B) Confocal images of frozen sections stained with antibodies to β-catenin (red), LGR5 (green) or the DNA stain DAPI (blue). All images are Z-stacks of confocal sections. For each set, the upper panel shows the three combined stains, the middle panel LGR5 (greyscale), and the bottom panel β-catenin (greyscale). Images were obtained on a Nikon C1 confocal microscope with a 60× oil lens. C) Specificity control for LGR5 staining: frozen sections were stained with antibody to β-catenin and normal rabbit serum, followed by Alexa 488 anti-rabbit Ig (green) and Alexa 546 anti-mouse Ig (red) and the nuclear stain DAPI (blue). The green channel (NRS) and red channel (β-catenin) are shown separately in greyscale. Images were obtained as in B).