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. 2011 Jul 28;6(7):e22733. doi: 10.1371/journal.pone.0022733

Figure 8. Expression of junctional proteins in Lim 1899 cells with altered LGR5 expression.

Figure 8

Cells transfected with empty vector, siRNA to LGR5 (siLGR5) or pTune/LGR5 (LGR5) were grown in chamber slides and prepared for immunofluorescence as described in Methods. Slides were stained with antibodies to E-cadherin (A), β-catenin (B) or Zo-1 (C) followed by Alexa 488 anti-mouse Ig (green channel in all samples). Cells were counterstained with rhodamin-phalloidin (red channel) and the nuclear stain DAPI (blue channel). Images are Z-stacks of sequential confocal images. For each antibody, the top panel shows the merged channels and the bottom panel the green channel only (grey scale).