Table 1. Changes in wnt pathway gene expression upon modulation of LGR5 in LIM1899 cells.

Gene	Fold changeLGR5 silencing	p value	Fold changeLGR5 overexpression	p value
WISP1*	8.74	0.03	−1.60	0.57
Wnt 5a*	7.38	0.004	−3.53	0.04
Casein Kinase 1γ*	4.05	0.04	−1.21	0.84
FGF4*	3.41	NA	−6.33	NA
Axin 1	2.26	0.07	−2.43	0.10
Frizzled 7*	2.02	0.004	−2.55	0.39
Lef -1*	−4.39	0.03	1.76	0.05
FRAT 1	−3.20	0.03	2.28	0.13
DIXDC1	−2.82	0.11	3.14	0.016
DAAM 1	−2.52	0.11	2.00	0.19
TLE2*	−2.38	0.02	1.89	0.21
Tcf 7 *	−2.25	0.25	2.01	0.18
CTNNBIP1	−2.16	0.05	3.29	0.01
Kremen 1*	−2.24	0.02	1.55	0.15
CtBP1	−2.05	0.04	1.45	0.15
CtBP2 *	−2.52	0.05	2.16	0.08

Expression levels of wnt pathway genes were analysed in three independent samples for each of parental cells, cells with LGR knockdown and cells with LGR5 overexpression using SABioscience pathway arrays. Data are presented as fold-change relative to the parental cells. Data were analysed as described in Methods using the SABioscience analysis program. Only genes with at least 2-fold change in either sample set, and for which there are significant differences between the two sample sets, are reported.

*the same trend in gene expression was observed in LIM1215 after knockdown of LGR5.