. 2011 Jul 28;6(7):e22810. doi: 10.1371/journal.pone.0022810

Table 1. P. brasiliensis identified proteins with increased expression during iron starvation.

Spot¹	Time²	GenBank gi³	Protein identification	PMF score	MS/MS score	MW⁴	pI ⁵	≥fold change⁶
METABOLISM
Nucleotide metabolism
1	24 h	gi\|226280544	Adenosine kinase	193	49	36.6/41.3	5.4/5.5	1.3
2	24 h	gi\|226283962	GMP synthase	133	-	60.3/55.2	6.2/7.4	3.6
C-compound and carbohydrate metabolism
3	6 h	gi\|295664927	ATP-citrate-lyase	159	303	52.8/46.6	5.9/6.9	1.2
Amino acid, nitrogen and sulfur metabolism
4	24 h	gi\|226280080	2-nitropropane dioxygenase	163	107	38.8/41.9	5.4/5.1	^**
5	24 h	gi\|27368050	Formamidase	108	100	46.1/46.1	6.1/7.1	1.3
6	24 h	gi\|226284927	Hydroxyacylglutathione hydrolase	66	86	28.9/33.1	6.1/7.1	1.7
7	24 h	gi\|226282479	L-threonine 3-dehydrogenase	105	129	38.0/40.3	5.8/6.4	1.9
8	24 h	gi\|226293104	Spermidine synthase	93	32	33.6/36.0	5.3/5.1	1.6
Lipid, fatty-acid and isoprenoid metabolism
9	24 h	gi\|226279101	Carbonic anhydrase	90	104	32.6/27.4	9.1/8.2	1.8
Phosphate metabolism
10	6 h	gi\|295672504	Inorganic pyrophosphatase	141	67	33.3/38.0	5.1/4.8	1.3
ENERGY
Electron transport and membrane-associated energy conservation
11	24 h	gi\|226282053	ATP synthase subunit beta	199	118	55.1/33.3	5.2/5.0	2.5
Glycolysis and gluconeogenesis
12	24 h	gi\|146762537	Enolase	110	173	47.4/63.4	5.6/6.2	^**
13	24 h	gi\|29826036	Fructose 1,6-biphosphate aldolase	163	174	39.7/41.1	6.1/7.1	2.6
14	6 h	PAAG_01995	Fructose 1,6-biphosphate aldolase	77	-	39.6/40.3	6.0/6.8	1.4
15	24 h	gi\|226279559	Glucokinase	113	49	55.7/52.2	5.3/5.0	2.8
16	24 h	gi\|226285327	Phosphoglycerate kinase	108	72	45.3/43.4	6.4/7.3	1.6
17	24 h	gi\|295669690	Phosphoglycerate kinase	149	72	45.3/44.1	6.4/7.3	1.8
18	6 h	gi\|295669690	Phosphoglycerate kinase	82	53	45.3/42.9	7.8/7.7	1.2
19	6 h	gi\|295670663	Triosephosphate isomerase (Tpi)	185	87	27.1/30.3	5.3/5.6	1.4
Tricarboxylic-acid pathway
20	24 h	gi\|295673931	Pyruvate dehydrogenase protein X component	110	41	52.7/50.6	6.4/5.4	1.5
21	24 h	gi\|226280161	Pyruvate dehydrogenase protein X component	100	131	52.7/50.3	6.4/5.3	1.4
CELL CYCLE AND DNA PROCESSING
22	24 h	gi\|225683196	Tubulin alpha-2 chain	105	-	50.5/52.4	5.0/4.7	1.2
23	24 h	gi\|225683196	Tubulin alpha-2 chain	117	57	50.5/53.2	5.0/4.9	^**
24	24 h	gi\|226285902	Tubulin beta chain	146	65	50.3/51.1	4.8/4.5	1.8
25	24 h	gi\|154705473	Septin-1	123	87	44.1/44.3	5.2/5.1	2.0
26	24 h	gi\|226294796	Actin	78	59	38.1/43.5	7.1/5.7	1.6
27	24 h	gi\|38569374	14-3-3-like protein 2	120	84	29.7/37.1	4.6/4.4	1.7
28	24 h	gi\|226282286	DNA damage protein rad24 (14-3-3 protein)	147	252	32.4/40.1	4.7/4.2	1.4
TRANSCRIPTION
29	24 h	gi\|226280907	mRNA binding post-transcriptional regulator (Csx1)	92	44	42.6/44.2	6.3/6.9	2.0
30	24 h	gi\|226284577	Type 2A phosphatase activator tip41	90	-	35.9/27.9	5.3/4.9	2.3
31	6 h	PAAG_06891	mRNA binding post-transcriptional regulator (Csx1)	96	-	42.4/43.4	6.3/7.1	2.0
PROTEIN SYNTHESIS
32	24 h	gi\|226280659	60S ribosomal protein L5	104	-	34.3/39.7	9.0/9.7	^**
33	24 h	gi\|226282202	ATP-dependent RNA helicase eIF4a	130	60	45.0/45.1	5.1/4.8	1.3
34	24 h	gi\|226280705	Elongation factor 2	103	110	92.6/37.8	6.4/7.2	^**
35	24 h	gi\|226283670	Translation initiation factor eIF3	84	56	66.0/62.3	5.1/5.2	^**
PROTEIN FATE (folding, modification, destination)
36	6 h	PAAG_05417	Mitochondrial-processing peptidase	140	-	53.0/45.2	5.8/7.1	1.3
37	6 h	gi\|295661059	G-protein complex beta subunit cpcb	102	228	35.4/38.5	6.5/7.9	3.3
CELL RESCUE, DEFENSE AND VIRULENCE
38	24 h	gi\|60656557	Heat shock protein 90	148	91	80.3/73.7	4.9/5.0	1.4
39	24 h	gi\|226285144	Hsp90 co-chaperone AHA1	150	48	36.5/42.0	5.4/5.7	1.4
40	24 h	gi\|31324921	Heat shock protein SSC1 (70 kDa)	135	225	73.8/67.1	5.9/5.1	1.5
41	24 h	gi\|295659116	Hsp70-like protein	244	126	70.9/42.7	5.0/5.7	1.6
42	24 h	gi\|226286087	Mitochondrial peroxiredoxin PRX1	197	97	24.8/29.2	5.2/4.8	1.2
43	24 h	gi\|17980998	Y20 protein	92	82	21.6/15.5	6.0/8.5	2.2
UNCLASSIFIED PROTEINS
44	24 h	gi\|226278304	Conserved hypothetical protein	103	40	38.0/38.5	6.0/7.2	1.4
45	24 h	gi\|226286114	Conserved hypothetical protein	92	114	34.7/40.6	5.3/4.9	1.6
46	24 h	gi\|226279849	Conserved hypothetical protein	86	75	11.8/13.0	5.0/4.3	^**
47	6 h	PAAG_06617	Conserved hypothetical protein	113	-	30.5/34.9	6.2/7.1	1.3

**Spots visualized only in iron-depleted condition;

Spots numbers refers to Figure 3;

Time in iron starvation condition;

GenBank general information identifier;

⁴

Molecular weight (theoretical/experimental);

⁵

pI (theoretical/experimental).

⁶

≥fold in iron depletion.