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. 2011 Jul 28;6(7):e22810. doi: 10.1371/journal.pone.0022810

Table 1. P. brasiliensis identified proteins with increased expression during iron starvation.

Spot1 Time2 GenBank gi3 Protein identification PMF score MS/MS score MW4 pI 5 ≥fold change6
METABOLISM
Nucleotide metabolism
1 24 h gi|226280544 Adenosine kinase 193 49 36.6/41.3 5.4/5.5 1.3
2 24 h gi|226283962 GMP synthase 133 - 60.3/55.2 6.2/7.4 3.6
C-compound and carbohydrate metabolism
3 6 h gi|295664927 ATP-citrate-lyase 159 303 52.8/46.6 5.9/6.9 1.2
Amino acid, nitrogen and sulfur metabolism
4 24 h gi|226280080 2-nitropropane dioxygenase 163 107 38.8/41.9 5.4/5.1 **
5 24 h gi|27368050 Formamidase 108 100 46.1/46.1 6.1/7.1 1.3
6 24 h gi|226284927 Hydroxyacylglutathione hydrolase 66 86 28.9/33.1 6.1/7.1 1.7
7 24 h gi|226282479 L-threonine 3-dehydrogenase 105 129 38.0/40.3 5.8/6.4 1.9
8 24 h gi|226293104 Spermidine synthase 93 32 33.6/36.0 5.3/5.1 1.6
Lipid, fatty-acid and isoprenoid metabolism
9 24 h gi|226279101 Carbonic anhydrase 90 104 32.6/27.4 9.1/8.2 1.8
Phosphate metabolism
10 6 h gi|295672504 Inorganic pyrophosphatase 141 67 33.3/38.0 5.1/4.8 1.3
ENERGY
Electron transport and membrane-associated energy conservation
11 24 h gi|226282053 ATP synthase subunit beta 199 118 55.1/33.3 5.2/5.0 2.5
Glycolysis and gluconeogenesis
12 24 h gi|146762537 Enolase 110 173 47.4/63.4 5.6/6.2 **
13 24 h gi|29826036 Fructose 1,6-biphosphate aldolase 163 174 39.7/41.1 6.1/7.1 2.6
14 6 h PAAG_01995 Fructose 1,6-biphosphate aldolase 77 - 39.6/40.3 6.0/6.8 1.4
15 24 h gi|226279559 Glucokinase 113 49 55.7/52.2 5.3/5.0 2.8
16 24 h gi|226285327 Phosphoglycerate kinase 108 72 45.3/43.4 6.4/7.3 1.6
17 24 h gi|295669690 Phosphoglycerate kinase 149 72 45.3/44.1 6.4/7.3 1.8
18 6 h gi|295669690 Phosphoglycerate kinase 82 53 45.3/42.9 7.8/7.7 1.2
19 6 h gi|295670663 Triosephosphate isomerase (Tpi) 185 87 27.1/30.3 5.3/5.6 1.4
Tricarboxylic-acid pathway
20 24 h gi|295673931 Pyruvate dehydrogenase protein X component 110 41 52.7/50.6 6.4/5.4 1.5
21 24 h gi|226280161 Pyruvate dehydrogenase protein X component 100 131 52.7/50.3 6.4/5.3 1.4
CELL CYCLE AND DNA PROCESSING
22 24 h gi|225683196 Tubulin alpha-2 chain 105 - 50.5/52.4 5.0/4.7 1.2
23 24 h gi|225683196 Tubulin alpha-2 chain 117 57 50.5/53.2 5.0/4.9 **
24 24 h gi|226285902 Tubulin beta chain 146 65 50.3/51.1 4.8/4.5 1.8
25 24 h gi|154705473 Septin-1 123 87 44.1/44.3 5.2/5.1 2.0
26 24 h gi|226294796 Actin 78 59 38.1/43.5 7.1/5.7 1.6
27 24 h gi|38569374 14-3-3-like protein 2 120 84 29.7/37.1 4.6/4.4 1.7
28 24 h gi|226282286 DNA damage protein rad24 (14-3-3 protein) 147 252 32.4/40.1 4.7/4.2 1.4
TRANSCRIPTION
29 24 h gi|226280907 mRNA binding post-transcriptional regulator (Csx1) 92 44 42.6/44.2 6.3/6.9 2.0
30 24 h gi|226284577 Type 2A phosphatase activator tip41 90 - 35.9/27.9 5.3/4.9 2.3
31 6 h PAAG_06891 mRNA binding post-transcriptional regulator (Csx1) 96 - 42.4/43.4 6.3/7.1 2.0
PROTEIN SYNTHESIS
32 24 h gi|226280659 60S ribosomal protein L5 104 - 34.3/39.7 9.0/9.7 **
33 24 h gi|226282202 ATP-dependent RNA helicase eIF4a 130 60 45.0/45.1 5.1/4.8 1.3
34 24 h gi|226280705 Elongation factor 2 103 110 92.6/37.8 6.4/7.2 **
35 24 h gi|226283670 Translation initiation factor eIF3 84 56 66.0/62.3 5.1/5.2 **
PROTEIN FATE (folding, modification, destination)
36 6 h PAAG_05417 Mitochondrial-processing peptidase 140 - 53.0/45.2 5.8/7.1 1.3
37 6 h gi|295661059 G-protein complex beta subunit cpcb 102 228 35.4/38.5 6.5/7.9 3.3
CELL RESCUE, DEFENSE AND VIRULENCE
38 24 h gi|60656557 Heat shock protein 90 148 91 80.3/73.7 4.9/5.0 1.4
39 24 h gi|226285144 Hsp90 co-chaperone AHA1 150 48 36.5/42.0 5.4/5.7 1.4
40 24 h gi|31324921 Heat shock protein SSC1 (70 kDa) 135 225 73.8/67.1 5.9/5.1 1.5
41 24 h gi|295659116 Hsp70-like protein 244 126 70.9/42.7 5.0/5.7 1.6
42 24 h gi|226286087 Mitochondrial peroxiredoxin PRX1 197 97 24.8/29.2 5.2/4.8 1.2
43 24 h gi|17980998 Y20 protein 92 82 21.6/15.5 6.0/8.5 2.2
UNCLASSIFIED PROTEINS
44 24 h gi|226278304 Conserved hypothetical protein 103 40 38.0/38.5 6.0/7.2 1.4
45 24 h gi|226286114 Conserved hypothetical protein 92 114 34.7/40.6 5.3/4.9 1.6
46 24 h gi|226279849 Conserved hypothetical protein 86 75 11.8/13.0 5.0/4.3 **
47 6 h PAAG_06617 Conserved hypothetical protein 113 - 30.5/34.9 6.2/7.1 1.3

**Spots visualized only in iron-depleted condition;

1

Spots numbers refers to Figure 3;

2

Time in iron starvation condition;

3

GenBank general information identifier;

4

Molecular weight (theoretical/experimental);

5

pI (theoretical/experimental).

6

≥fold in iron depletion.