Skip to main content
. 2011 Jul 28;6(7):e22836. doi: 10.1371/journal.pone.0022836

Figure 7. Upregulation of HYAL1 increased tumor growth, angiogenesis, HAase activity and HA expression of MCF7 cells in vivo.

Figure 7

Representative mouse bearing tumors, the average tumor volume (A) from 5th weeks and tumor weight (B) in MCF7-HYAL1 group were increased significantly than in MCF7-Vec group (p<0.01). Western blot (C) and immunohistochemistry (D) analysis showed that the expression of HYAL1 protein was enhanced in the MCF7-HYAL1 group obviously, but HYAL2 levels were not altered. Compared with MCF7-Vec, expression of ki67 and CD31 were increased in MCF7-HYAL1 (D). ELISA-like assay measured HAase activity (E) and HA levels (F) present in the tissue extracts, the HAase activity and HA levels in MCF7-HYAL1 were higher than MCF7-Vec (p<0.01, p<0.05, respectively).