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. 2011 Jul 28;6(7):e22838. doi: 10.1371/journal.pone.0022838

Figure 4. miR-96 inhibits γ-globin expression in human erythropoiesis.

Figure 4

(A,C,E) Cord blood-derived erythroid cultures (CB) were transduced with miRNA precursors; (B,D,F) Adult bone marrow-derived erythroid cultures (BM) were transduced with anti-miRNAs. Cells were harvested and analyzed at day 8 (d8), 11 (d11) and 14 (d14). (A–B) Specific amounts of γ-globin per total protein concentration and (C–D) relative amounts of γ-globin compared to negative control, which was set to 100%, were measured by ELISA. Values represent mean ± SEM of 3 experiments (n = 3). P values were determined by the Student's t-test. * p<0.05. (E–F) Representative cytospins of erythroid cells on d8, d11 and d14. The cytospins were stained with May Grünwald Giemsa and images were acquired with a Zeiss Axioskop2 microscope equipped with a Zeiss Plan-Apochromat 63×/1.4 oil immersion objective lens and a Zeiss AxioCam MRc digital camera. Images were recorded using Zeiss AxioVision AC release 4.5.0 software.