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. 2011 Jul 28;6(7):e22879. doi: 10.1371/journal.pone.0022879

Figure 2. Analysis of the binding and kinase-activating properties of different Cyclin A2 alleles in NIH3T3 cells.

Figure 2

WT, D171A, Triple (M200A, L204A and W207A), M200A, L204A, W207A (A), and WT, E180A, E210Q, Double (W207L, E210Q), K256A, L282R and E285A Cyclin A2 alleles (B). Immunoprecipitation using anti-Flag-agarose affinity gel was performed on NIH3T3 cells extracts after transfection of plasmids encoding the above-mentioned mutants. Immune complexes were either submitted to Western blotting to identify binding partners or analyzed for their histone H1-kinase activity. Schematic representation of the importance of the different Cyclin A2 domains for partners association and kinase-induced activity. The bars represent (high-medium-low according to their size) the importance of the indicated region of Cyclin A2 for its association with partners or kinase inducing activity (C).